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Weifeng Shang

Weifeng Shang, Ph.D.

Weifeng Shang
Research Assistant Professor

Education 

Ph.D. Univerity of Muenster, Germany
M.Sc. University of Siegen, Germany
B.Eng. Zhejiang University, China

Expertise 

Shang works mainly in the area of synchrotron radiation instrumentation, X-ray detectors, X-ray imaging, small-angle X-ray scattering (SAXS) using synchrotron radiation for biological macromolecules. Shang started to work in this area in 2001 when Shang completed a master's thesis on position sensitive gas-filled proportional counters and developed a time-resolved data acquisition system for such detectors. After a Ph.D. in experimental physics, Shang was a postdoc at the European Molecular Biology Laboratory biological SAXS beamline in Hamburg. Responsibilities focused on the hardware/software development of the synchrotron radiation beamline and the high-throughput SAXS capability for biological samples. Shang also collaborated with external structural biologists and biochemists on the SAXS data analysis and modeling. Shang also worked in X-ray medical imaging on mammography systems at GE Global Research Center for almost two years. Since April 2013, Shang joined the BioCAT beamline 18ID at the Advanced Photon Source, Argonne National Laboratory. Shang's work focuses on X-ray instrumentation and software development to enhance and extend the SAXS capability at BioCAT beamline in the area of size exclusion chromatography SAXS, simultaneous small- and wide-angle X-ray scatteing, and time-resolved micro-beam SAXS on the milli-second time scale using continuous flow turbulent mixers and laminar flow mixers.

Publications 

X-ray instrumentation Publications

  • W. Shang, B. Robrahn, F. Golding and M. H. J. Koch, A versatile data acquisition system for time resolved X-ray scattering using gas proportional detectors with delay line readout, Nuc. Instr. Meth. A 530 (2004) 513-520.
  • C. E. Blanchet, A. V. Zozulya, A. G. Kikhney, D. Franke, P. V. Konarev, W. Shang, R. Klaering, B. Robrahn, C. Hermes, F. Cipriani, D. I. Svergun and M. Roessle, Instrumental setup for high-throughput small- and wide-angle solution scattering at the X33 beamline of EMBL Hamburg, J. Appl. Cryst. (2012) 45 489-495

Small Angle X-ray Scattering Application on Biological Macromolecules Publications

  • W. Shang, I. Ivanov, D. I. Svergun, O. Y. Borbulevych, A. M. Aleem, S. Stehling, J. Jankun, H. Kühn and E. Skrzypczak-Jankun, Probing dimerization and structural flexibility of mammalian lipoxygenases by small-angle X-ray scattering, J. Mol. Biol. (2011) 409, 654-668.
  • I. Ivanov, W. Shang, L. Toledo, L. Masgrau, D. I. Svergun, S. Stehling, H. Gómez, A. Di Venere, G. Mei, J. M. Lluch, E. Skrzypczak-Jankun, À. González-Lafont and H. Kühn, Ligand induced formation of transient dimers of mammalian 12/15-Lipoxygenase: a key to allosteric behavior of this class of enzymes?, PROTEINS: Structure, Function, and Bioinformatics (2012), 80, 703-712.
  • L. Brunotte, R. Kerber, W. Shang, F. Hauer, M. Hass, M. Gabriel, M. Lelke, C. Busch, H. Stark, D. I. Svergun, C. Betzel, M. Perbandt, S. Günther, Structure of the Lassa virus nucleoprotein revealed by X-ray crystallography, small-angle X-ray scattering, and electron microscopy. J. Biol. Chem. (2011) 286, 38748-38756.
  • F. Meersman, C. Atilgan, A. J. Miles, R. Bader, W. Shang, A. Matagne, B. A. Wallace and M. H. J. Koch, Consistent picture of the reversible thermal unfolding of Hen Egg-White Lysoyzme from experimental and molecular dynamics, Biophys. J. 99 (2010) 2255-2263.
  • I. Lorenzen, W. Shang, M. Perbandt, M. V. Petoukhov, D. I. Svergun, G. H. Waetzig, S. Rose-John, R. Hilgenfeld and J. Grötzinger, The structure of the unliganded extracellular domain of the interleukin-6 signal transducer gp130 in solution, Eur. J. Cell Biol. (2010) 90, 515-520. (contributed equally with first author).
  • T. Zou, X. Yao, B. Qin, M. Zhang, L. Cai, W. Shang, D. I. Svergun, M. Wang, S. Cui and Q. Jin, Crystal structure of Pseudomonas aeruginosa Tsi2 Reveals a Stably Folded Superhelical Antitoxin, J. Mol. Biol. (2012) 417, 351-361.
  • Y. Xiao, Q. Ma, T. Restle, W. Shang, D. I. Svergun, R. Ponnusamy, G. Sczakiel and R. Hilgenfeld, Nonstructural Proteins 7 and 8 of Feline Coronavirus Form a 2:1 Heterotrimer That Exhibits Primer-Independent RNA Polymerase Activity, J. Virol. 2012, 86:4444.
  • E. de Almeida Ribeiro, M. Beich-Frandsen, P. V. Konarev, W. Shang, B. Vecerek, G. Kontaxis, H. Hammerle, H. Peterlik, D. I. Svergun, U. Blasi and K. Djinovic-Carugo, Structural flexibility of RNA as molecular basis for Hfq chaperone function, Nucleic Acids Research, 2012, vol. 40, 8072-8084.
  • Z. Sayers, M. Aydin, E. Bal, F. Yesilirmak, P. V. Konarev, M. V. Petoukhov, M. Roessle, A. G. Kikhney, W. Shang and D. I. Svergun, Comparative Characteriztion of Apo-, Reconstituted- and In Vivo-Folded forms of a Durum Wheat Metallothionein, Biophysical Journal, 2013, vol. 104, 396A.
  • A. Bowman, C. M. Hammond, A. Stirling, R. Ward, W. Shang, H. El-Mkami, D. A. Robinson, D. I. Svergun, D. G. Norman and T. Owen-Hughes, The histone chaperones Vps75 and Nap1 form ring-like, tetrameric structures in solution, Nucleic Acids Research, 2014, vol. 42, 6038-6051.

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Memberships 

  • Sigma Xi

Grants 

9P41GM103622-20 (Irving, PI)
9/30/1997 – 12/31/15
NIH/NIGMS
"The Biophysics Collaborative Access Team"

  • The major goal of this project is to operate a Biotechnology Research Center at the Advanced Photon Source for non-crystalline diffraction scattering and x-ray spectroscopy of biological molecules. 

Role: co-investigator

1 S10 OD018090-01 (Irving, PI)
5/1/2014 to 4/30/2015
"Advanced Pixel Array Detector for Time-Resolved SAXS and Fiber Difraction"

  • The Major goal of this project was to obtain a high performance CCD-based X-ray Detector for synchrotron fiber diffraction applications.

Role: co-investigator